Developmental Cell, DOI: ( /j.devcel ) Copyright © 2011 Elsevier Inc. The merged image and the red channel (detecting red and yellow cells) are shown on the left and right, respectively (Hadjieconomou et al., 2011, adapted with permission). EGFP (green) is the default fluorescent protein, when Flp is not active. (E) An example of a mosaic in an 元 larval optic lobe, showing labeled lineages of medulla (m) and lamina (l) neurons, in blue, red, or yellow, after transient heat shock at early larval stages. Prior to expression the potential fluorescent marker is indicated as an outline full colored circles indicate expressing cells. Elav-Gal4155 drives expression of the UAS construct in neurons. (D) Generation of mosaics by heat-shock induction of the Flp recombinase, which induces stochastic labeling of cells. (C) In Drosophila, the Flybow construct (version FB1.1) of membrane tagged fluorescent reporters can be rearranged by the action of Flp on FRT sites. (B) Schematic illustration of cell labeling in the lineage. After nonmitotic recombination, cells become yellow (Zong et al., 2005 Griffin et al., 2009). If mitotic recombination takes place, green and red fluorescent reporters mark separate sister cells as a result of allelic segregation. (A) In the MADM system in mouse or in the twin-spot system in Drosophila, partial sequences for fluorescent proteins are separated by recombination sites (►), which, when exposed to recombinase, reconstitute a fluorescent reporter. Terms and ConditionsĤ Figure 3 Examples of Reporter Constructs for Mosaic Analyses (D) In the MAZe procedure, developed in zebrafish, transient activation of a heat-shock promoter (hsp) leads to expression of a reporter via the Gal4/UAS system (Collins et al., 2010). (C) In the Kaloop system, as used in zebrafish, autoactivation of the fluorescent marker provides permanent labeling after tissue-specific initiation of expression, without recombination (Distel et al., 2009). (B) Genetic inducible fate mapping (GIFM) used in mouse or G-TRACE in Drosophila depends upon activation of a ubiquitous reporter by a tissue-specific recombinase (Zinyk et al., 1998 Evans et al., 2009). (A) Schematic representation of the mode of action of such procedures. Terms and Conditionsģ Figure 2 Genetic Manipulations for Spatiotemporal Control of Reporter Expression (A–C) Schematic representation of genetic tracing procedures based on tissue-specific expression. (C) Schematic representation of the mode of action of this BAPTI system on cell lineage (Caron et al., 2008, adapted with permission). Terms and ConditionsĢ Figure 1 Live Imaging of Kaede Photoconversion in Zebrafish: An Example of Prospective Lineage Analysis (A) Photoconversion of the Kaede fluorescent protein by exposure to UV light induces rupture of a covalent bond (adapted with permission from (B) Early trigeminal sensory neurons born before the photoconversion appear yellow, due to the presence of the photoconverted red protein and the newly synthesized protein (green), whereas the neurons born later remain green. Meilhac Developmental Cell Volume 21, Issue 3, Pages (September 2011) DOI: /j.devcel Copyright © 2011 Elsevier Inc. Presentation on theme: "Tracing Cells for Tracking Cell Lineage and Clonal Behavior"- Presentation transcript:ġ Tracing Cells for Tracking Cell Lineage and Clonal Behavior
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